A P, Tettamanti G, Gagliano N, Anastasia L: Isolation andAbbah et

A P, Tettamanti G, Gagliano N, Anastasia L: Isolation andAbbah et al. Stem Cell Research Therapy bmjopen-2016-011824 2014, 5:38 http://stemcellres.com/content/5/2/Page 3-Bromo-5-chloro-2-fluoroaniline 9 of138. 139.140.141.characterization of 2 new human rotator cuff and long head of biceps tendon cells possessing stem cell-like self-renewal and multipotential differentiation capacity. Am J Sports Med 2013, 41:1653?664. Lutolf MP, Gilbert PM, Blau HM: Designing materials to direct stem-cell fate. Nature 2009, 462:433?41. Keung AJ, Kumar S, Schaffer DV: Presentation counts: microenvironmental regulation of stem cells by biophysical and material cues. Annu Rev Cell Dev Biol 2010, 26:533?56. Huang TF, Yew TL, Chiang ER, Ma HL, Hsu CY, Hsu SH, Hsu YT, Hung SC: Mesenchymal stem cells from a hypoxic culture improve and engraft PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/744568 achilles tendon repair. Am J Sports Med 2013, 41:1117?125. Cigognini D, Lomas A, Kumar P, Satyam A, English A, Azeem A, Pandit A, Zeugolis D: Engineering in vitro microenvironments for cell based therapies and drug discovery. Drug Discov Today 2013, 18:1099?108.10.1186/scrt426 Cite this article as: Abbah et al.: Assessment of stem cell carriers for tendon tissue engineering in pre-clinical models. Stem Cell Research Therapy 2014, 5:
Leyh et al. Stem Cell Research Therapy 2014, 5:77 http://stemcellres.com/content/5/3/RESEARCHOpen AccessOsteoarthritic cartilage explants affect extracellular matrix production and composition in cocultured bone marrow-derived mesenchymal stem cells and articular chondrocytesMichaela Leyh1,2, Andreas Seitz3, Lutz D selen3, Hans-Robert Springorum1, Peter Angele4, Anita Ignatius3, Joachim Grifka1 and Susanne Gr sel1,2*AbstractIntroduction: In the present study, we established a novel in vitro coculture model to evaluate the influence of osteoarthritis (OA) cartilage explants on the composition of newly produced matrix and chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells (BMSCs) and the phenotype PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/9547713 of OA chondrocytes. In addition, we included a "tri-culture" model, whereby a mixture of BMSCs and chondrocytes was cultured on the surface of OA cartilage explants. Methods: Gene expression analysis, protein and glycosaminoglycan (GAG) assays, dot-blot, immunofluorescence, and biomechanical tests were used to characterize the properties of newly generated extracellular matrix (ECM) from chondrocytes and chondrogenically differentiated BMSCs and a mix thereof. We compared articular cartilage explant cocultures with BMSCs, chondrocytes, and mixed cultures (chondrocytes and BMSCs 1:1) embedded in fibrin gels with fibrin gel-embedded cells cultured without cartilage explants (monocultures). Results: In general, co- and tri-cultured cell regimens exhibited reduced mRNA and protein levels of collagens I, II, III, and X in comparison with monocultures, whereas no changes in GAG synthesis were observed. All co- and tri-culture regimens tended to exhibit lower Young's and equilibrium modulus compared with monocultures. In contrast, aggregate modulus and hydraulic permeability 2,2,3,3-Tetrafluoropropyl N,N'-diethylcarbamimidothioate trifluoromethanesulfonate seemed to be higher in co- and tri-cultures. Supernatants of cocultures contained significant higher levels of interleukin-1 beta (IL-1), IL-6, and IL-8. Stimulation of monocultures with IL-1 and IL-6 reduced collagen gene expression in BMSCs and mixed cultures in general but was often upregulated in chondrocytes at late culture time points. IL-8 stimulation affected BMSCs only. Conclusions: Our results suggest an inhibitory effect of OA cartilage on the.